The wild rice species Oryza meyeriana Baill exibited high resistance or immunity to bacterial blight (BB). Two new germplasms, SH5 and SH76, were acquired through somatic hybridization with O. meyeriana L. as the donor parent. Using 11 BB races, the resistance spectrum of SH5, SH76 and other 16 reference lines carrying different major BB resistance genes were compared at the tillering and booting stages. The results showed that SH5 and SH76 had a broad resistance spectrum. The resistance spectrum of SH5 and SH76 was the same as IRBB21 (carrying Xa21), and similar to IRBB5 (carrying xa5), IRBB7 (carrying Xa7) and Asominori (carrying Xa17). The molecular marker pTA248 and XA21 were used to detect whether Xa21 existed in SH5 and SH76. The allelic test of Xa17 was conducted by crossing SH5 and SH76 with Asominori, respectively. The results combined with those of our previous analysis indicated that no Xa5, Xa7, Xa17 and Xa21 but a new resistance gene might exist in SH5 and SH76.