Morphological traits and growth habits of F. viridis Duch. and five accessions of F. viridis self-cross progeny were described. Variation among five self-cross accessions and F. viridis was assessed, of all 67 biological traits, we obtained soluble solids and other fourty two significant differences. Fifteen obvious traits were conducted by principal component analysis and variation coefficient analysis, plant height, plant size, pedicle length, pedicle thickness, petiole thickness, inflorescence thickness and pollen vitality provide the key indicator for diversity of five self-cross accessions and F. viridis. A significant difference of self-cross fruit setting rate was found for this five accessions and Ls-S1-4 was considered as highly self-incompatibility. The research will lay the foundation for self-compatiblity/incompatibility lines.