The full length cDNA sequence of chalcone synthase gene (CHS) from Abelmoschus esculentus was isolated by homologous sequences cloning and RT-PCR. This sequence is 1175bp, and it predicts an open reading frame of 1170bp corresponding to 389 amino acids, which is designated as AeCHS. Bioinformatics analysis shows that the deduced AeCHS amino acid sequence has the higher homology with Abelmoschus manihot and Gossypium hirsutum, respectively 99.23% and 97.44%. The deduced AeCHS amino acid sequence contains a chalcone synthase family tag sequence(GFGPG) and four conserved active sites: Cys164, Phe215, His303, Asn336. The expression analysis of AeCHS gene in different growth period of okra fruits, flowers, leaves are carried out by RT-qPCR and the results indicate that AeCHS gene shows different expression patterns: flower > fruit > leaf. Specific to different plant tissues, the expression of AeCHS gene reachs a higher level in fruits grown six days, blooming flowers and the fourth leaves numbering from the top of plants.