The genetic diversity and phylogenetic relationship of 20 pumpkins landraces of Zhejiang province were investigated by 82 phenotypic traits and cluster analysis. The results showed that the variation coefficient of the 36 quantitative traits varied between 7.33% and 59.11%, and Shannon-weave genetic diversity coefficient of the 46 qualitative traits ranged from 0 to 1.99, implying the abundant genetic diversity. The cluster analysis based on the phenotypic traits indicated that 20 pumpkins landraces were divided into two categories, and this was agreement with the traditional classification. One category of 19 pumpkins landraces could be further divided into five groups at Euclidean distance of 15.0. The divisions were correlated with the size of sepals, pedicel hairy, melon shapes and skins, and the divisions criterion were independent of each other. The results of this study enriched the evaluation system and provided important information for the exploitation and utilization of excellent gene resources of pumpkins.