The intergeneric hybrids of broccoli and radish were obtained by embryo rescue and identified by morphological characterization, cytological observation and molecular marker techniques. The results showed that the hybrids showed stronger growth vigor compared with their parents. Most phenotypic characters of the hybrids were beyond two parents. Cytological observation revealed that the hybrid plants possessed male sterility characteristic as female parent broccoli. According to SSR molecular marker analysis, the hybrids contained genetic information of both parents, which proved that hybrids were real hybrids. The creation and identification of the hybrids provide basic materials for producing new radish germplasm and useful bridge for gene communication between Brassica and Raphanus.