LFY gene plays an important role in the transformation process from vegetative growth to reproductive growth in flowering plants. Feijoa sellowiana, a promising fruit, was introduced to China recently. To clarify the expression and pattern of the FsLFY (F. sellowiana LFY) gene, the spatial and temporal expression pattern of the FSLFY gene was revealed by real-time fluorescent quantitative PCR (qPCR) technology, and the promoter sequences of the FsLFY gene sequences were isolated by the chromosome walking technology. QPCR analysis showed that the FsLFY gene was expressed at all developmental stages of floral buds and all different tissues/organs. In developmental buds, the expression level of the FsLFY was the highest in small bud stage and the lowest in middle bud stage. In tisses/organs, the expression level was the highest in stems from vegetative branches and the lowest in sepals. The newly cloned FsLFY promoter was 2436 bp in length and was deposited in GenBank under accession no. KF766536. Promoter analysis with online softwares PLACE and PlantCARE showed that the FsLFY promoter contained not only CAAT-box, TATA-box and other core promoter elements, but also the unknown cis-regulatory elements response to the water, light, and gibberellin(GA), implying that the expression of the FsLFY gene is regulated by various external environment conditions. This study would provide atheoretical basis for fully understanding the flowering mechanism of F. sellowiana and accelerating flowering and fruit setting via molecular breeding strategy.