The resistances of 166 soybean cultivars to prevalent strains were evaluated using manual inoculation method. These cultivars were selected from Huang-Huai-Hai and the strains were SC3, SC7, SC6, SC11 and the mix of the four. The results showed that, there were 82 cultivars resistant to the four strains, accounting for 49.4%; Among them, 32 cultivars immune or high resistance to the three strains and 23 cultivars immune to all the four strains, accounting for 19.3% and 13.9%, respectively. There were 108 cultivars resistant to the mix of the four strains, accounting for 65.1%. Among them, there were 45 immune and 29 high resistant, accounting for 27.1%, 17.5%, respectively. 62 cultivars resistant to the four strains and the mixed strains, taking up 37.3%, while 14 cultivars immune or high resistance to them, taking up 8.4%. The results also showed that, the resistance of the recently bred varieties was significantly enhanced than the early bred varieties to SC3 and SC7. Cultivars from Hebei, Beijing, Shanxi are more resistant to the strains with the lower disease index. Jidou9 and Shidou6 were immune to all strains and can be used as resistance resources in breeding programs. It is also demonstrated that some cultivars showed resistance difference after inoculated with the four and the mixed strains, indicating that there were significant interactions between strains.