Two hundred and forty inbred lines were identified and evaluated for the resistance to banded leaf and sheath blight (BLSB) under artificial inoculation conditions from 2010 to 2012.Meanwhile, the correlation between the resistance to BLSB and major agronomic traits were also analysis. The results showed that there were no immune nor highly resistant lines, four inbred lines with moderately resistant to BLSB, and 236 lines were sensitive to BLSB, among which 218 lines were highly sensitive to BLSB. Meanwhile, there were no resistance lines in Reid, Lvdahonggu, PA and Tangsipingtou genetic groups, however, Lancaster and PB genetic groups were identified to be important sources for maize BLSB resistance. Additionally, the results of correlation analysis showed that five traits, such as plant height, ear height, ear height / plant height, internodes number below the ear and internodes length below the ear, were significantly negative correlated with the disease index. These traits can be used as the reference for screening germplasm with resistance to BLSB without artificial inoculation.