Allele-specific PCR (AS-PCR) was adopted to molecular evaluation to 330 hybrids from 4 hybridization combinations, which only one male parent was high oleate peanut line CTWE and female parents were 4 normal oleate conventional varieties. The results of agarose gel electrophoresis showed only 230 hybrids had a specific 539 bp band. Hybridization success rates of Baisha 1016 ?CTWE, Haihua1 ?CTWE , Ji 0212-2 ?CTWE and Yuanza9847 ?CTWE were 83.3 %, 50.0 %, 57.1 % and 50.0 % respectively. The Near-Infrared Spectroscopy analysis (NIRS) for single kernal was adopted to measure the F2:3 family lines of Yuanza9847 ?CTWE ,Baisha 1016 ?CTWE, Ji 0212-2 ?CTWE and Haihua1 ?CTWE. High oleic family lines were 9,8,2 and 3 respectively, which deduced the ratio of FAD2B-m/FAD2B-m in F2 population was 9.47 %, 4.17 %, 3.39 % and 3.37 % respectively. The genetic pattern of the high oleic trait of 4 hybridization combinations all accorded with 2 pairs of genes at p=0.05 level . This study has certain reference value for the chromosomal identification for high oleic trait and also could increase the efficiency of high oleate peanut breeding.