Physical biolistic particle approach is one of the main techniques used for wheat genetic transformation. Efficient biolistic transformation system is benefit to the development of new wheat varieties, identification of candidate genes, and functional genomics study. In this paper, the main factors including genotypes and explants of wheat, plant growth regulators, bombardment parameters, selection system, etc, which affect wheat transformation efficiency mediated by biolistic bombardment, were reviewed, and the purpose is to provide some useful information for improving the protocol and efficiency of biolistic particle mediated transformation in wheat.