Abstract: 66 hybrids (F1) were got by crossing 12 selected typical inbred lines according to Griffing 4 model. The analysis of combining ability and genetic parameters were conducted for Pb2+ contents in leaves and kernels under 3 Pb2+ contamination levels via pot cultivating method in the greenhouse. According to the results, there were significant differences between general combining ability and special combining ability of Pb2+ content in maize leaves and kernels, and the non-addictive variance was larger than the addictive variances. The genetic variance was larger than the environmental variance, as well as the broad-sense heritability was larger than the narrow-sense heritability, indicating that this trait’s variance is mainly from genetic factors with a higher hereditary capacity. During the germplasm screening process, when the soil Pb2+ concentration was lower than 333.32 mg/kg， Using combinations crossed by parent strain Zheng58 would focus on not only the Pb2+ content of kernels in standard, but also the high enrichment of Pb2+ content in leaves, the main purpose was to conduct bioremediation for Pb2+ contamination in soil while taking feed and food security into account. When the Pb2+ concentration was higher than 715.46 mg/kg, using combinations crossed by parent strain 178 should focus on selecting germplasm of low Pb2+ accumulation in leaves and kernels, and this would offer some certain guidance for screening maize varieties in Pb2+ contaminated soil in the future and selecting breeding strategy of avoiding pollution.