The full sequence of GmMYB174 was isolated from soybean, including 1086 bp and encoding 361 amino acids. GmMYB174 belonged to MYB-related family. Bioinformatics analysis indicate GmMYB174 share a high similar with tomato, alfalfa and peanut, containing 2 conserved locus and a motif of SHAQKFF. GmMYB174 located in nucleus. The expression predication of tissue show that GmMYB174 expressed in many tissues and peaked in epicotyl. Promoter analysis indicate that the region of GmMYB174 promoter contain ABRE, MYB, MYC, LTRE and GT-1. Q-RT-PCR shows that GmMYB174 involved in various abiotic stresses. GmMYB174 maybe participated in multi-environmental stress responses.