Abstract: Wheat substitution lines between Chinese Spring and Synthetic 6x under the treatments of control(150mmol.L-1 NaCl) and salt stress(150mmol.L-1 NaCl)were studied to research the effect of salt stress on the changes of Na , K content, soluble sugar content, soluble protein content，proline content and to locate the genes controlling the relative characters. The results showed that contents of K and Na increased under salt treatment comparing to control. The organic osmotic substance of wheat substitution lines also increased significantly under salt tolerance. Chromosome location of relative characters showed that the genes inhibiting Na content might be located on 1A, 2A, 3B, 7B, 1D, 4D and 7D chromosome and increasing K content might be exist on 4A, 7A chromosome of Synthetic 6x, genes promoting soluble sugar content might be located on 6A, 7A, 7D chromosome and increasimg soluble protein content might be exist on 1A, 2A, 4A, 6A chromosomes and the genes promoting proline content might be located on 7A, 6D chromosome of Synthetic 6x. In sum, the fourth,seventh chromosomes (4A, 4D; 7A, 7D) Synthetic 6x may contain the genes regulated inorganic substances and the sixth chromosome (6A, 6D) may contain organic osmotic regulation genes.