To study drought resistance of different types of flax germplasm in germination, screen varieties with resistance to drought, and give support in breeding under drought conditions, this paper studies stress test under different concentrations PEG-6000 stress condition for Dingya 17, Longya 8 and 2011621080, to select the best concentration of PEG-6000 solution, for 161 varieties from northwest arid area of resistance to adversity crop germplasm resources survey of flax seed during germination, and meanwhile 5 relevant character which were relevant to drought resistance were studied. Comprehensive evaluation was carried out of drought resistance for different germplasm resources through drought resistance coefficient, comprehensive drought resistance coefficient, membership function and grey relational analysis. The results showed that had an influence on the germination of flax germplasm with the increased of PEG-6000 concentration, with 18% PEG-6000 stress condition for the flax germplasm of seed during germination among them, the relative germination rate, root length, root fresh weight, bud length, bud fresh weight of 161 flax germplasm decreased under PEG-6000 stress. According to drought resistance comprehensive evaluation value, flax germplasm used in this study were divided into 5 drought resistance grades, 2 belonged to grade 1, 15 belonged to grade 2, 28 belonged to grade 3, 82 belonged to grade 4, 34 belonged to grade 5. The capacity of drought resistance of the tested cultivars was relevant to geographical origins. Using several relevant characters, it is an effective and accurate way to comprehensively evaluate the drought tolerance of flax in germination.