In higher plant, Dof (DNA-binding with one finger) transcription factor is a plant-specific transcription factor, which plays important roles during the growth and development. Here, the genes of DcDofD1, which encoding to a Dof transcription factor, were cloned from two carrot varieties ‘Heitianwucun’ and ‘Junchuanhong’, respectively. The sequences of nucleic acid and amino acid, phylogenetic tree, hydrophilicity and hydrophobicity, and molecular modeling were analyzed using bioinformatics method. The results demostrated that the two DcDofD1 factors from carrot had single zinc finger, and their amino acid sequence at Dof domain were very conserved. The DcDofD1 factors from carrot were classified into D1 subfamily of Dof family. The expression profiles of the DcDofD1 gene under four abiotic stress treatments (cold, heat, high salt, and drought) were analyzed by quantitative real-time PCR in the two carrot varieties ‘Heitianwucun’ and ‘Junchuanhong’. The results of expression profiles showed that DcDofD1 gene responded to the cold, heat, high salt, and drought stresses in carrot. The results demostrated that the DcDofD1 gene involved into interact with abiotic stress in carrot.