Four PAL (phenylalanine ammonia-lyase) genes were cloned from Sumai3, which were designed as TaPAL1, TaPAL2, TaPAL3, TaPAL4. Their Open reading frames(ORFs) were 2142bp, 2016bp, 2118bp and 2139bp, and the length of the deduced protein were 714, 672,706 and 713 amino acid residues, respectively. Sequence alignment of the four genes showed the similarity was 88.35% and the encoded amino acids was 91.92%. Amino acids sequence analysis showed that the four proteins all contain HAL-PAL domain and PAL domain. Expression pattern analysis revealed that all the four PAL genes were up regulated after inoculation with Fusarium graminearum, which suggested that PAL genes might have an important role in the defense response to F. graminearum.