The genetic linkage map of wheat was constructed with DH population consisting of 289 individuals from a cross between BS20 and Fu3. 1112 SSR and EST-SSR primers were used to detect the polymorphisms between parents. Applying QGA_CN software to carry out linkage analysis ,the genetic map contains 19 chromosomes (without 4D and 6A) which included 128 SSR and 6 EST-SSR markers. The linkage map covered 2749.2 cM and the average distance was 17.4 cM. All 19 linkage groups consisted of 2-15 markers,the coverage of length from 15.3 to 244.4 cM. At the same time,we constructed three DNA pools including Beijing sterile pool,Fuyang sterile pool and restorer pool for screening the sterile genes using BSA method,and these primers were Wmc264, Wmc73 and Xgwm350,which distributed by chromosomes 3A, 5B and 2A/7D. Six major QTLs were detected when the F value>7.5 by mixed-model composite interval mapping.Thirteen major QTLs were found when the LOD value > 2.5 with composite interval mapping.Three QTLs were shared from two methods, the sterile genes were located on chromosomes 1BL , 2BS and 3AL, the marker intervals were Wmc365-cfa2129,Wmc602-Xgwm148 and Wmc264a-cfa2262 respectively,after the analysis of BSA and QTL.