In this paper, using state trial oil-tea variety (Camellia oleifera ‘Huashuo’) as material, a full-length cDNA of acyl-CoA dehydrogenase genes in seeds was cloned using RACE technique based on the transcriptome and expression profiling database of C. oleifera seeds. It was named as CoACAD (GenBank accession number KJ910338). The cDNA of CoACAD gene was found to be 2702 bp with an open reading frame of 2487 bp encoding 828 amino acid residues. The molecular mass of the CoACAD protein was 92.4113 kDa with theoretical pI of 8.47. CoACAD protein has two obvious transmembrane domains and contained a typical tyrosine protein kinase (PTK) active site, containing protein kinase domain, aminoglycoside phosphotransferase (APH) domain, ACAD_N domain, ACAD_C domain and ACAD Center domain. The expression vector of CoACAD were constructed successfully, and the BL21 (DE3) bacteria harboring pET30a-CoACAD was induced to express the recombinant protein which was about 93 kDa. The relative expression abundance of CoACAD at 13 different developmental stages of C.oleifera seeds was analyzed using real-time quantitative PCR (qPCR). The CoACAD gene are both up-regulated expression in the C.oleifera seeds’ enlargemental and mature period, which may play be involved in regulation of energy supply at different developmental stages of C. oleifera seeds.