Phytophthora root rot is one of the most important diseases of soybean and cause severe economic losses worldwide. Deployment of resistance or tolerance cultivars is the most effective method for controlling the disease. Screening of disease resistance resources is a basis of novel resistance genes or QTLs discovering and disease resistance breeding. In this study, resistance and tolerance to Phytophthora root rot in wild soybean germplasms were identified, in order to exploit novel resistance and tolerance sources. One hundred and four wild soybean accessions were identified for resistance to two isolates PS41-1 and PSJS2 of Phytophthora sojae using cotyledon plug-inoculation method in seedling. Thirty-three accessions were resistant to isolate PS41-1, 35 accessions were resistant to isolate PSJS2, and 18 accessions were resistant to the two isolates. Further, 82 accessions were selected for evaluation of tolerance to the two isolates of P. sojae using inoculum-layer method. Seven accessions had high levels of tolerance. These results indicated that elite sources of new P. sojae resistance and/or tolerance were present in wild soybean. The resistance and/or tolerance wild soybean maybe provide new sources for phytophthora root rot resistance breeding for diversifying resistance genetic base to Phytophthora root rot in soybean in the future.