Abstract: Multidrug and toxic compound extrusion (MATE) proteins, comprising the most recently designated family of multidrug transporter proteins, are identified, characterized and found to be involved in the detoxification of endogenous secondary metabolites and xenobiotics. Recent researches also showed that some of the MATE members play essential role in plant development and organ initiation. So far, the MATE family in common tobacco (Nicotiana tabacum) hasn’t been further studied. In this research, 131 MATE genes were preliminary identified in common tobacco by bioinformatics, and they were classified into 4 subfamilies. We also found that subfamily 3 was distinct from other subfamilies, including the conserved domain prediction, TM prediction and subcellular location prediction, there was the same situation in subfamily 3 in Arabidopsis. Many MATE genes showed low expression level in all tissues by transcript analysis. GO analysis indicated that as a kind of transporters, the MATE family members are involved in a series of biological processes such as stimulus responses and biological regulation. The results we got will be helpful for the further analysis of the MATE family proteins in other plants.