The investigation, collection and arrangement of agricultural biological resources related to work and life of the Miao national minority, Yi national minority, Bai national minority and Hui national minority were carried on in 18 villages distributed in 7 townships in Hezhang county in Guizhou Province on September and October 2014. The survey collected accessions of 239 agricultural biological resources, which include the 97 characteristic, special and high quality accessions. In this paper, the current situation of local agricultural biological resources, the reasons for the growth and decline, the types and the value use of collected resources were analyzed. The protection and the development of agricultural biological resources in Hezhang county were discussed.