Creation and screening of mutants are an important source of mining gene, and also are one of the effective ways to expand the germplasm resources. In this paper, indica rice cultivar 9311 seeds were treated with 60Co γ-ray, and 134 mutants were obtained by M2 generation screening and M3 generation identification, and the mutation frequency was 10.24%. The types of mutation included growth period and fertility, leaf traits, stem morphology, panicle traits, grain shape, and so on. Moreover, we also found a dwarf mutant with shorter internodes and leaves and without reproductive growth, and the mutant can not flower and fruit after continuously planting in Hubei and Hainan province. The mutants provide abundant materials for rice functional genomics study, and some of them can be directly used as breeding materials.