TTC (2,3,5-Triphenyl tetrazolium chloride) vitality staining conditions for potato shoot tips of Huzi83-213cultivar were optimized in this study. The optimal staining temperature and duration were determined as 40ºC and 2h, respectively. The viability of potato shoot tips was investigated during droplet-vitrificaion cryopreservation by TTC staining under the optimal conditions. The fresh shoot tips and precutured (Murashige and Skoog liquid medium with 0.3 mol/L and 0.5 mol/L sucrose) shoot tips showed high vitality. However, after cryoprotection with plant vitrification solution No.2 (PVS2 solution), the shoot tips had the temporal and spatial specificity of viality loss and survival, and the inner meristem and the middle part of the leaf primordium kept high vitalities. The results between TTC staining viality and survival percentages after recovery showed extremely significant positive correlation (P<0.01)when the area ratio of the red-stained shoot tips was higher than 0.4.