Abstract: Identification of the resistance to TuMV of 127 Brassica campestris L. ssp. chinensis germplasm was conducted with the method of artificial inoculation identification and ELISA test respectively. The result showed that the DI value ranged between 3.55 and 95.68, which expressed significant difference of resistance to TuMV among accessions. Distribution plot for different resistance level to TuMV basically corresponds to normal distribution and the peak was slightly partial to susceptible region. The result of cluster analysis based on DI value was similar to that of the grading levels. 6 accessions highly resistant to TuMV and 13 accessions resistant to TuMV were screened by seedlings inoculation identification. Their main agronomic characters demonstrate abundant diversity. The number of resistance accessions with the characters of having wrinkling or pubescence on leaf surface takes a relatively higher ratio, which demonstrates that the leaf character might have a certain correlation with TuMV resistance. The result of ELISA test showed that the amount of virus in 117 materials was significantly different with P/N value ranging between 3.10 and 25.37, but it had no significant correlation with DI value. ELISA test could be used as a supplementary identification method in screening resistance materials.