In this work, pot experiment was conducted in Beijing to study the differences in biomass and its allocation of 14 switchgrass. The results showed that the low-land switchgrass Kanlow produced the most biomass with dry weights of stems, aboveground parts and the whole plant reaching to 175.48 g/plant, 299.18 g/plant and 447.66 g/plant, respectively. While, the up-land switchgrass Nebraska produced the least biomass with dry weights of stems, aboveground parts and the whole plant being 20.53 g/plant, 58.08 g/plant and 140.51 g/plant, respectively. Taking the whole plant into consideration, Kanlow allocated the highest proportion (63.13%) of biomass into aboveground parts compared to the other switchgrass cultivars, while the lowest (40.55%) was found in Canada. Moreover, Kanlow allocated the highest proportion (48.67%) of biomass into aboveground vegetative organs and the lowest proportion (31.88%) was found in Nebraska. Taking aboveground parts of switchgrass as a whole, Alamo, Kanlow and Trailblazer allocated higher proportion of biomass (35.91%, 37.09% and 34.39%, respectively) into stalks and higher stems/leaves ratio (2.75, 2.56 and 2.48, respectively) was found compared with other switchgrass cultivars. The origin latitude significantly influenced the switchgrass biomass and its allocation. Considering the whole switchgrass plant, there was significant negative correlation between the origin latitude and switchgrass biomass. Significant positive correlations between the origin latitude and biomass proportion of belowground parts and the significant negative correlations between origin latitude and the biomass proportion of aboveground parts, seeds as well as stems were also found. When talking about aboveground parts of switchgrass, a significant negative correlations between origin latitude and stems biomass proportion as well as the stems/leaves ratio were found, while significant positive correlations exhibited between origin latitude and leaves and sheaths biomass proportion. The differences of biomas and its allocation reflected a reproductive and growth strategy long-term adaptive to the ecological environment in switchgrass. This work provides the basis for introduction of genetic resources and the variety breeding of switchgrass.