In order to explore the excellent germplasm resources from Yunnan tartary buckwheat germplasm resources, broaden the genetic basis of tartary buckwheat, six agronomic traits and five quality traits of 48 taytary buckwheat genotypes in Yunnan had been analyzed for genetic diversity. The results showed that the abundant genetic diversity existed among the tartary buckwheat in Yunnan, the variation coefficient of seed weight per plant in agronomic traits was 34.4% largest, and the variation coefficient of total flavonoids in quality traits was 66.7% largest. The results of the cluster analysis indicated that 48 accessions could be classified into 3 clusters including the low yield type, shorter plant height with high yield type and middle plant height with high yield type. The principal components analysis based on six agronomic traits and five quality traits, indicated the accumulation indices of respectively 84.105% and 80.332% from the top three principal components which reflecting the breeding potential of major quantitative traits. Comprehensive analysis of main traits provided scientific information for effective use of tartary buckwheat hermplasm resources in Yunnan genetie resources.