Abstract: It is a general trend to grow cotton in saline alkali arid area in order to solve the contradiction between grain and cotton. Therefore, it is of great significance to carry out the identification and screening of resistant germplasm .Salt tolerance of 179 glandless cotton germplasm resources was identified by using germination box and quartz sand in the incubation room. The results showed that no high salt resistant variety was found. However, 4 resistant, 54 tolerant and 121 sensitive varieties were discriminated, accounting for 2.2%, 30.2% and 67.6% in the glandless cottons, respectively. The better salt resistant and tolerant materials are Yuwu424, Hengwu87-306, Zhongwu1651, Zhongwu3385, Duomao101, Zhongwu374-G, Zhongwu1038 and Zhongwu642. The relative survival rate of seedlings of these materials was above 70%under salt stress. For the sources of the cottons, salt tolerance of domestic materials was better than that of foreign materials. Salt tolerance of materials from Hebei, Shandong and Henan provinces was better than that of materials from other provinces. In this study, we selected good glandless cotton materials with good salt resistance, which provided excellent germplasm resources for the breeding of glandless cotton.