Three pathogenic samples of Plasmodiophora brassicae from Changyang in Hubei Province, Taibai in Shanxi Province, and Xinye in Henan Province were used for inoculation to evaluate the clubroot disease resistance of 22 cabbage materials, respectively. The homologous genes of CRa and Crr1a in Brassica oleracea genome were analyzed by sequence homology analysis and then were amplified, sequenced and alignment for analysis. The results showed that 22 cabbage materials showed different resistance spectrum to three P. brassicae samples, indicating that three pathogenic samples were different physiological races. Screened resistant materials BDH3, Chou hybride Tekila, SW-110, CGL-8, Syngenta varieties, SW-109 could be used as clubroot-resistant breeding materials and mining new resistance gene in the future. Three genes (Bo7g107710, Bo7g107730 and Bo7g107740) on chromosome 7 of B. oleracea genome were identified as homologues of CRa, and the DNA sequence of Bo7g107730 gene showed relatively high variation in resistant material SW-110, which may be related to clubroot resistant. In addition, only one gene Bo3g164040 homologue to Crr1a were identified on chromosome 3 of B. oleracea genome, and we speculated that this gene was not resistant to clubroot disease because of this gene showed high sequence identity between resistant and susceptible material.