The Trinity program was used to assemble the transcriptome sequences of eggplant. In total，45 404 Unigenes were obtained with the length of 4 791 066 bp. We detected 8 316 SSR loci from these Unigenes by using MISA software. The density of the SSR were 5.63 kb with the frequency of 18.32%. The single nucleotide SSR is the most abundant （5 372） type which count for 64. 60% the total loci and followed by the tri-nucleotide （1 628） for 19.58%. The tri-nucleotide repeat motifs of AAG/CTT was the predominant repeat types that accounting for 31.6% of the tri-nucleotide repeat motifs. The dinucleotide repeat motifs of AG/CT is the predominant repeat format in this types of repeat and accounting for 42.3%. A total of 858 pairs of SSR primers are designed by using primer3 online program and 100 pairs of SSR markers are randomly selected and verified by using 17 eggplant germplasms. Among them，84 pairs of primers are able to amplify PCR products， of which 47 pairs of primers produced polymorphic bands. The PIC values range was between 0.10 to 0.64，the average value was 0.32. The 17 eggplant germplasms could be divided into three phylogenetic groups by analysis with UPGMA method. The results indicated that the SSR markers based on the transcriptome sequencing in eggplant will provide more reliable markers for map structure，analysis of genetic polymorphism for eggplant.