Sixty tea germplasm from Qiannan prefecture were collected to construct DNA fingerprint database by SSR technique. The results showed that 147 alleles were detected with considerable polymorphism by 15 pairs of primers employed in the experimental design.The ranges of expected heterozygosities and polymorphism information content at 15 SSR loci of 60 cultivars were 0.128-0.939 and 0.124-0.927 respectively，with an average of 0.602 and 0.572 respectively.And a total of selected 23 alleles from 5 primers (QNSSR01、QNSSR02、QNSSR04、QNSSR06、QNSSR18、QNSSR23) were used for the DNA fingerprint construction. Sixty cultivars with different SSR fingerprints of each other can serve as the cultivars-specific patterns and as an important basis for cultivars identification.