Using automatic DNA analyzer multiple SSR-PCR fluorescent marker detection technology, we analyzed the genetic diversity of 259 maize germplasms and 11 kind of inbred lines through core 50 standard test simple sequence repeat loci which cover whole genome. Among 50 SSRs, the total No. allele was 214 for 270 inbred lines. The average of the gene diversity and PIC was 0.50 and 0.44, respectively. The 270 assays could be divided into 3 groups by UPGMA clustering method. Furthermore, we analysis the numerical value of heterosis group, in order to make clear the application of germplasm resources. The results in this study could provide the genetic basis for improving the efficiency of hybrid breeding.