In order to define the distribution characteristics of dwarfing genes in wheat varieties in qinghai province and excavate the less use of dwarfing genes, this study used eight specific molecular markers of wheat dwarfing genes to test the dwarfing genes in 85 wheat varieties in qinghai province. Results showed that more than 56.1% of 82 varieties in qinghai wheat materials (50) contained dwarfing genes, with which Rht-B1b and Rht-8 gene frequency was higher, at 28.04% and 23.17% respectively. Only eight materials contained Rht-D1b gene, 11 materials contained Rht5 gene and 9 materials contained Rht12 gene, at 9.76%、13.41%、9.76% respectively. In the varieties containing the dwarfing gene, there were 18 materials containing more than 2 and 2 dwarfing genes accounting for 21.95% of the total materials. There were two varieties containing three dwarfing genes at the same time, including one containing Rht-B1b、Rht8 and Rht12 genes, one containing Rht-B1b、Rht5 and Rht8 genes. Five varieties contained Rht-B1b and Rht8 genes at the same time.Two materials contained Rht-D1b and Rht8 genes at the same time. Five materials contained Rht-B1b and Rht5 genes an the same time .Two materials contained Rht8 and Rht5 genes at the same time. One material contained Rht-D1b and Rht5 genes at the same time. And the rest of the 32 varieties each contained one dwarf gene. No variety was found at the same time containing Rht- B1b, Rht-D1b genes.