liu chunliang
Institute of Crop Sciences, ChineseAcademy of Agricultural Scienceswanglanfen
Institute of Crop Sciences, ChineseAcademy of Agricultural Scienceswujing
Institute of Crop Sciences, ChineseAcademy of Agricultural Scienceswangshumin
Institute of Crop Sciences, ChineseAcademy of Agricultural SciencesGrowth habit is an important trait associated with the domestication in common bean(Phaseolus vulgaris L.),which is a component trait including the types of inflorescence and stem.To locate the growth habit gene, we established F2 plants and F2:3 plants families derived from a cross between indeterminate viny common bean cultivar LiannongziyunNO.1(LianziNo.1) and determinate bush landrace Tuzitui (F0404).The genetic analysis showed that determinate bush was controlled by a single recessive gene, designed as gh-lz, which was primarily mapped on B1 linkage group by bulked segregant analysis. With a lager mapping population, more SSR and In/Del markers, the gh-lz gene was further mapped between SSR marker p1t52 and In/Del marker In93, sited on 45,453,003bp-45,575,103bpon B01, with a physical region about 122kb. This region included 12 genes, named Gene1~Gene12.The 12 candidategenes were annotated by BLAST searching NCBI, and Gene12 was annotated as TFL1 in common bean.These results provided a foundation for growth habit trait study and gene cloning.