GXAS07-6-1, an intergeneric hybrid of E. arundinaceus and S. spontaneum L. has the advantages of its parents, which make it an important material for sugarcane innovation breeding.In this study, The F1 hybrids GXAS08-2-17, GXAS08-2-22 and GXAS08-2-32 were identified as a true progenies of E.arundinaceus by the method of Alu-like. And the chromosomes composition of the GXAS07-6-1 and the F1 generation were analyzed using the genomic in situ hybridization (GISH) technique. The results showed that there were 62 chromosomes in GXAS07-6-1,of which 30 are from E. arundinaceus and 32 are from S.spontaneum. The somatic chromosome karyotypic types of GXAS07-6-1 is 1B , following n n transmission. Furthermore, there were 75 to 84 chromosomes in the F1 hybrids, of which 78 to 80 are from Saccharum spp and S. spontaneum GXS79-9, and 9 to 11 are from E. arundinaceus. The somatic chromosome karyotypic types of 3 F1 progeny were 2B, 1B, 1B, respectively, following n n transmission. Chromosome translocation and exchange were not observed in the intergeneric hybrid GXAS07-6-1 and it’s F1 hybrids as well . We also confirmed the occurrence of chromosome missing from E. arundinaceus.in the F1 progeny.