A total of 290 inbred lines with rich genetic diversity were used in this study. We investigated the relationship between Stalk pushing resistance and fiber quality related traits with at different densities. Stalk pushing resistance and fiber quality related traits excellent performance lines were screened by multiple comparisons among different heterosis. The results shows that different lines had highly significant difference at different densities, furthermore , significantly positive correlation was studied between stalk pushing resistance and content of cellulose. The results of multiple comparison shows that content of cellulose among different heterosis no significant difference at high density. Pushing resistance exist significant difference under two density and content of cellulose under low density among different heterosis. In different heterosis, the number of lines of screened out with excellent performance about pushing resistance and content of cellulose under two density, and there are 4 and 3 lines in the Reid group, 1 and 5 lines in the Lancaster group, 2 and 2 lines in the P group, 2 and 1 lines in the LRC group, 2 and 1 lines in the TangSPT group.