Mung bean [Vigna radiata (L.) Wilczek] is one of the most important grain legumes in China. Fusarium wilt, caused by the fungal pathogen Fusarium oxysporum, is a severe soil-borne disease on mung bean. F. oxysporum firstly infect roots of plants, and caused symptoms including leaf chlorosis and necrosis, vascular discoloration of roots and stems. Lastly, the plants of mung bean infected seriously died. Deployment of resistant cultivars is the most economic and effective way to control this disease. In this study, the mung bean cultivars with contrasting resistant phenotype were used as plant materials, and the factors affecting resistance phenotype including inoculation method, plant development stage, inoculum concentration, inoculum treatment time and incubation temperature for inoculated plants, were comparatively analyzed. The results showed that the root dipping technique is the optimal inoculation method. The seedling of mung bean at 2 leaf age was suitable to inoculation with pruned roots dipped in spore suspension of 105~106 spore/mL for 2 min. Inoculated plants should be incubated at 25℃. The survey time of disease scoring can effectively shorten experimental period at 14 days after inoculation. This method is rapid and effective for evaluation of mung bean resistance to Fusarium wilt in controlling condition, which will provide technical support for mung bean germplasm resistance screening and breeding program.