58 seedlings were obtained from the cross of Victoria cruziana with V. ‘Longwood Hybrid’ offspring, and the characteristics segregation of seedlings was studies and seedlings were clustered. The results showed that: the segregation of seedlings characteristics was wide range and show mid-parent heterosis and over-parent heterosis, there is transgressive inheritance in 6 characteristics; there were higher correlations in the first night inner petals color, the first night stamenoid color, the second night outer petals color, the second night inner petals color and the second night inner-outer shading, and lower correlations in rims color, buds type, sepals color, number of thorns on sepals and the first night outer petals color which most liked independent inheritance. The plants characteristics types were diversified, could be divided into 7 categories which were green leaves and outer pink-inner white flowers type, red leaves and outer fushcia-inner white flowers type, green leaves and outer fushcia-inner deep fushcia flowers type, red leaves and outer fushcia-inner deep fushcia flowers type, green leaves and outer deep fushcia -inner white flowers type, red leaves and outer pink-inner deep fushcia flowers type, green leaves and thorny sepals and outer fushcia-inner white flowers type.