Drought is one of the important problem for Job’s tears production. Identification, indices screening, and cultivar breeding of drought resistance in Job’s tears germplasm resources are important for Job’s tears industrial development. The germination rates of 6 Job’s tears germplasms with different drought resistance were tested under 5 different concentrations of PEG-6000 water solution to determine the optimal osmotic potential of PEG-6000 water solution simulated drought stress at germination stage. The results showed that -0.1 MPa (80 g/L) was the optimum osmotic potential of PEG-6000 water solution simulated drought stress at germination stage for Job’s tears germplasm resources. The changes of germination energy, germination rate, germination index, bud length, bud diameter, bud fresh weight, bud dry weight, root length, root diameter, root fresh weight, and root dry weight of 50 Job’s tears germplasms were investigated under -0.1 MPa PEG-6000 water solution simulated drought stress to identify the drought resistance and screen the drought resistance indices of Job’s tears germplasm resources by comprehensive evaluation method at germination stage. The results showed that drought stress had significant effects on all indices of Job’s tears at germination stage. yy18-1, yy14-3, and yy13-1 were identified as drought resistance Job’s tears germplasms at germination stage, which could be provide basis materials for the researches on cultivar breeding, mechanism, and regulation and alleviation mechanism of drought resistance in Job’s tears. The bud length, bud dry weight, root length, root fresh weight, and root dry weight could be used as the simple and intuitive identification indices of drought resistance in Job’s tears germplasm resources at germination stage.