Sorghum plays an important role in white wine industry and agriculture supply-side reform. Grain tannin and color are two important agronomic traits, but molecular genetic mechanisms of these two traits were not clear, and there were no cloned genes refered to grain color in sorghum to now. 325 recombinant inbred lines were constructed with BTx623 (white grian and low tannin) and Rio (pink grain and high tannin). Combination of two years phenotype data, 118 simple sequence repeat (SSRs) and 8 insertion-deletion (INDELs) markers, and QTL IciMapping4.1 were used in mapping with inclusive composite interval mapping (ICIM) method. 3 and 6 QTLs were mapped, which related to content of grain tannin and seed color, respectively. QTL qTan4-1 was controlled by a known gene Sb04g031730, and the other two QTLs, qTan1-1 (txp11 - txp279, 1.29%) and qTan2-1 (txp298 ~ SB1292, 1.28%) were new in content of grain tannin QTL analysis. In grain color analysis, qGC1-1 (1.00% and 12.03%), qGC1-2 (2.64% and 16.96%), qGC1-3 (22.81%), qGC2-1 (1.00%), qGC2-2 (1.19%) and qGC6-1 (15.08%) were mapped between txp11 - txp279, txp43 - txp11, 1d1A3- sam44127, txp72-txp298 and txp298-SB1292, txp57- sam43683 (chr6), repectively. Our study paves a way to fine mapping grain tannins and color, gene cloning and marker-assisted selection breeding.