Soil salinization seriously impacts wheat (Triticum aestivum L.) production. Improving salinity tolerance is one of the main approaches responding to the problem. Salinity-tolerant germplasm resources provide a plant material basis for the improvement of salt tolerance in crops. The present research firstly selected the appropriate salt stress conditions for evaluating salinity tolerance at germination and seeding stages by screening a series of NaCl concentrations. The results showed that 1.2% NaCl solution and 0.8% NaCl in soil were the optimal concentrations for germination and seeding stages separately. Under the selected NaCl stress conditions, some morphological and physiological traits of 321 wheat genotypes were tested at the two stages. The salinity tolerance of the genotypes were evaluated and classified by principal component analysis and cluster analysis. Total of 21 and 18 genotypes with high salinity tolerance at germination and seeding stages were identified, which accounted for 6.5% and 5.6% of the tested genotypes, respectively. Two genotypes, Zhongzuo 60115 and Jimaiyihao, were determined as the high salinity tolerance at both germination and seeding stages. There was no significant correlation between D values of salinity tolerance at germination and seedling stages.