Verticillium wilt seriously influences the yields and qualities of eggplant. Studies found that extensive collection, identification and screening of the resistant materials especially from the wild resources, was the preferred option to solve the harms of Verticillium wilt. In Yunnan province, it is rich in wild eggplant resources, but these resources haven’t been utilized effectively. In this study, 3 purified isolates from infected plants were collected from the main production area of Yunnan Province, and they were determined as Verticillium dahliae Kleb. based on morphological and molecular identifications. Afterwards, one of the strains (QZ-S) was selected by resistance evaluation and to be used in the following inoculation experiment. The results found that, among 45 Yunnan wild eggplant resources that were inoculated with strain QZ-S, 2 materials ( Solanum sisymbrifolium and S. aculeatissimum ) showed high disease resistance, 2 materials ( S. torvum and S. chrysotrichum ) showed disease resistance and 6 materials ( including 1 S. violaceum material and 5 S. integrifolium materials) showed moderate resistance, and a high susceptibility material (239-3-2) was selected for its high disease percentage and disease index. All of the materials that were selected in this study could be appropriately utilized as disease resistance breeding resources. Besides, this study could broaden the germplasm resource of eggplant, lay the foundation for shortening the breeding period and providing the resistent resources for eggplant and the other crops.