Analysis of Protein Content and Genetic Polymorphism in Pea Germplasm in Tibet
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XiZang Agiricultural And Animal Husbandry College,XiZang Agiricultural And Animal Husbandry College,XiZang Agiricultural And Animal Husbandry College,XiZang Agiricultural And Animal Husbandry College

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    Abstract:

    Fifty-four landraces of field pea from different ecological and geographical environment conditions of Tibet were collected for this study. Total seed protein content, water soluble and salt soluble protein content of above genotypes of field pea were examined. While, the relationships between the protein contents (total seed protein content, water soluble and salt soluble protein content) of above genotypes and geographical factors (longitude, latitude and elevation) were analyzed. The results showed that the total protein content of 54 pea seed samples ranged from 17.58% to 28.67%, among which water soluble proteins accounted for 86.12 to 91.40%, and salt soluble proteins accounted for 4.76 to 8.29%. The total protein content of Tibet field pea seed samples significantly correlated with longitude, and correlated with latitude, but negatively correlated with altitude. 1588 water soluble and 699 salt soluble protein bands were detected by SDS-PAGE electrophoresis; among them, 43 band types of water soluble protein and 24 band types of salt soluble protein based on migration rate were identified with diversity index ranged from 0 to 0.5. The relative molecular weight of water soluble proteins ranged from 24.87Ku to 149.54Ku, showing concentrated areas of low molecular weight (24.71~50.41Ku) and high molecular weight (56.34-88.08ku). The relative molecular weight of salt soluble proteins ranged from 24.85Ku to 91.24 Ku. The 54 pea landraces is divided into 4 geographical groups according to the altitude information, with genetic diversity indices of 0.23, 0.18, 0.35 and 0.31, and Shannon information index of 0.33, 0.41, 0.52 and 0.46. The results showed that the pea protein variation related to altitude. Cluster analysis divided the 54 field pea landraces into 7 groups, indicating that water soluble and salt soluble

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History
  • Received:July 03,2017
  • Revised:July 16,2017
  • Adopted:August 01,2017
  • Online: March 19,2018
  • Published:
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