Abstract: Leaf rust is an important wheat disease which has a great influence on wheat yield. Breeding durable resistant cultivars can effectively and economically control the disease. The objective of this study is to identify leaf rust resistance genes in wheat cultivar Laizhou137 by gene postulation, pedigree analysis, molecular detection and adult plant resistance identification. The cultivars were tested for seedling responses in the greenhouse to 20 Puccinia triticina pathotypes and for adult plant to a mixed pathotypes (FHRT, THTT, THJT) in the field in 2014-2015 and 2015-2016 cropping seasons in Baoding, Hebei Province and Zhoukou, Henan Province. Meanwhile, pedigree method verifyed the results of known resistance genes. CIMMYT line SAAR, with typical slow rusting resistance and Zhengzhou5389, a highly susceptible line were used as slow rusting and susceptible checks, respectively. Differential sets containing 36 differential lines mostly in a background of Thatcher with known leaf rust resistance genes were used to compare the infection types of the cultivar at seedling stage, the 12 molecular markers of ten leaf rust resistance genes were used for the tested cultivar. Gene postulation combined with markers detection and pedigree analysis, Laizhou137 showed low ITs to races (FGBQ、PGJQ、TGTT、THSM、PHGM、PHST、FHJS、FHGQ、FNTQ、PRSQ and KHGQ), thus it indicated that Laizhou137 contained Lr26, Lr10, Lr14b and other unknown resistance genes; According to the leaf rust resistance phenotype data in the field through four environments, it had unknown adult-plant resistance gene for expressing slow rusting resistant and having no Lr34 and Lr46 detected by molecular marker. Therefore, it can be new resistant resource for wheat leaf rust.