CIPKs, Calcineurin B-like (CBL) interacting protein kinases, belong to a group of serine/threonine protein kinases in plants. We cloned TaCIPK8 gene (GenBank number: KJ561804) from wheat variety Shi4185 based on the gene sequence of Arabidopsis AtCIPK8, and the length of TaCIPK8 coding sequence is 1350 bp. Sequence analysis indicated that TaCIPK8 protein consists of 449 amino acids, and its molecular mass and theoretical isoelectric point are 52.24 kD and 7.16, respectively. The protein contains an N-terminal kinase domain and a C-terminal NAF/FISL motif specifically possessed by the CIPK family proteins; the similarity betweenTaCIPK8 and AtCIPK8 protein is 81%. In order to investigate the function of TaCIPK8 gene, we detected the responses of TaCIPK8 gene to different stress conditions using Real-time PCR, and found that the expression of TaCIPK8 gene was induced by high salinity, ABA and low temperature (4℃). We analysed the promoter of TaCIPK8 gene using plant promoter databases PlantCARE and PLACE. The results indicated that the promoter harbors not only many cis-acting elements responsive to dehydration, drought, low temperature and ABA, but also DNA elements responsive to GA and jasmonic acid methyl ester. Yeast two hybrid was used to study the interaction between TaCIPK8 and TaCBLs proteins, and we found that the yeast transformed with both TaCIPK8 containing vector and TaCBL3 containing vector could grow on SD/-Trp/-Leu、SD/-Trp/-Leu/X-α-Gal/AbA and SD/-Trp/-Leu/-Ade/-His/X-α-Gal/AbA media, with blue colonies on the latter two media. The results indicate that the TaCIPK8 protein interacted with TaCBL3, which activated the expression of the reporter genes MEL1, AUR1-C, HIS3 and ADE2. Our results provide useful information for revealing the functions of TaCIPK8 gene and the interaction regulatory network between the protein of TaCIPK8 and TaCBLs in wheat.