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Home > Archive>Volume 19, Issue 3, 2018 >468-477. DOI:10.13430/j.cnki.jpgr.2018.03.012
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Analysis of genetic diversity, origin and evolution of Sweetpotato(Ipomoea batatas(L.)Lam.) by SRAP
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Crops Research Institute,Fujian Academy of Agriculture Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture,Crops Research Institute,Fujian Academy of Agriculture Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture,Crops Research Institute,Fujian Academy of Agriculture Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture,Crops Research Institute,Fujian Academy of Agriculture Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture,Crops Research Institute,Fujian Academy of Agriculture Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture,Crops Research Institute,Fujian Academy of Agriculture Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture,Crops Research Institute,Fujian Academy of Agriculture Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China,Ministry of Agriculture,

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    Abstract:

    The objective of this study is to make the genetic analysis and origin of sweet potato (Ipomoea batatas (L.) Lam.) by using sequence-related amplified polymorphism (SRAP) markers. 129 sweet potato accessions including wild relatives, landraces and cultivars were used. The phylogenetic trees of sweet potato and its wild relatives were constructed by MEGA and DPS softwares, and the genetic distance of sweet potato accession was calculated. The results showed that: (1) the average genetic distance of 124 cultivars and 5 wild relatives was 0.1848 and 0.4822, respectively. (2) The wild relatives were separated from cultivars at the level of L1=0.31, and 124 cultivars were classified into 8 different groups at the level of L2=0.21. (3) 129 sweet potato accessions were placed into 3 groups (I, II and III) on the SRAP constructed phylogenetic tree. Group I included 5 wild relatives, and group II contained 1 landrace and 6 cultivars. There were 117 cultivars and landcaces in the group III. (4) Being consistent with previous publication, the divergence time of the wild relatives was the longest, followed by local accessions, whereas cultivars were the shortest. Moreover, the divergence time of Fujian landraces and the cultivars from Philippines was same, indicating the evolution pathway of sweet potato from The Philippines to Fujian province of China is believable. In conclusion, SRAP is a useful method for deciphering the genetic diversity and origin of sweet potato germplasm.

    Reference
    [1] 王红意, 翟红, 王玉萍, 等. 30个中国甘薯主栽品种的RAPD指纹图谱构建及遗传变异分析[J]. 分子植物育种, 2009,7(5):879-884
    [2] 范泽民.中国甘薯品种30年[J].中国种业, 2015,8:6-9
    [3] 陆国权. 中国甘薯育成种系谱[J]. 中国甘薯, 1990,(4):26-28
    [4] 赵冬兰, 唐君, 曹清河, 等. 中国甘薯地方种质资源遗传多样性分析[J]. 植物遗传资源学报, 2015,16( 5): 994-1003
    [5] 江苏徐州甘薯研究中心. 中国甘薯品种志[D]. 北京: 农业出版社, 1993
    [6] Fu Y B, Peterson G, Diederichsen A, Richards K W. RAPD analysis of genetic relationships of seven flax species in the genus Linum L[J]. Genetic Resources and Crop Evolution, 2002, 49: 253-259.
    [7] 何余堂, 陈宝元, 傅廷栋, 李殿荣, 涂金星. 白菜型油菜在中国的起源与进化[J]. 遗传学报, 2003, 30(11): 1003-1012.
    [8] 盖钧镒, 许东河, 高 忠, 等. 中国栽培大豆和野生大豆不同生态类型群体间遗传演化关系的研究[J]. 作物学报, 2000, 26(5): 513-520.
    [9] Cheng Z, Lu B R, Sameshima K, Fu D X, Chen J K. Identification and genetic relationships of kenaf (Hibiscus cannabinus L.) germplasm revealed by AFLP analysis[J]. Genetic Resources and Crop Evolution, 2004, 51: 393-401.
    [10] Bao Y, Ge S. Identification of Oryza species with the CD genome based on RFLP analysis of nuclear ribosomal ITS sequences[J]. Acta Botanica Sinica, 2003, 45(7): 762-765.
    [11] Roy A, Bandyopadhyay A, Mahapatra A K, Ghosh S K, Singh N K,Bansal K C, Koundal K R, Mohapatra T. Evaluation of genetic diversity in jute (Corchorus species) using STMS, ISSR and RAPD markers[J]. Plant Breeding, 2006, 125(3): 292-297.
    [12] Kochert G, Stalker H T, Cimenes M, Galgaro L, Lopes C R, Moore K. RFLP and cytogenetic evidence on the origin and evolution of allotetraploid domesticated peanut, Arachis hypogaea (Leguminosae). American Journal of Botany, 1996, 83(10): 1282-1291.
    [13] 陶爱芬, 祁建民, 李木兰, 等. SRAP 结合ISSR 方法分析黄麻属的起源与演化[J]. 中国农业科学, 2012,45(1):16-25
    [14] 李强, 揭琴, 刘庆昌, 等. 甘薯基因组DNA高效快速提取方法[J]. 分子植物育种, 2007, 5(5):743-746
    [15] 李爱贤, 刘庆昌, 王庆美,等. 利用SRAP标记构建甘薯分子连锁图谱[J]．作物学报, 2010, 36(8):1286-129
    [16] Nei M , Li W H . Mathematical model for studying genetic variation in terms of restriction endonucleases [J] . Proceedings of National Academy of Sciences. USA, l979, 76: 5269-5273
    [17] 李亚玲, 韩国民, 何沙娥, 张智俊. 基于DNA 分子标记数据构建系统进化树的新策略[J]. 生物信息学, 2008, 4: 168-170.
    [18] Jarret R L, Austin D F, Genetic diversity and systematic relationships in sweetpotato (Ipomoea Batatas(L.)Lam.) and related species as revealed by RAPD analysis[J]. Genetic Resources and Crop Evolution, 1994,41:165-173
    [19] Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction：its application to mapping and gene tagging in Brassica[J]．Theor Appl Genet, 2001. 103:455-46l
    [20] 李强,刘庆昌,马代夫,等. 中国甘薯主要育成品种的遗传多样性及遗传趋势[J]. 江苏农业学报,2009,25(2):253-259
    [21] 蒲志刚,王大一,谭文芳,等. 南瑞苕AFLP指纹图谱的构建与聚类分析[J]. 西南农业学报,2007, 20(5):1085-1087
    [22] 贺学勤,刘庆昌,翟红,等. 用RAPD、ISSR和AFLP分子标记分析系谱关系明确的甘薯品种的亲缘关系[J]. 作物学报,2005,31(10):1300-1304
    [23] Ferriol M, Pico B, Cordava P F. Molecular diversity of a germplasm collection of sqash (Cacurbita moschata) determined by SRAP and AFLP makers[J]. Crop Sci, 2004,44:653-664
    [24] 陆漱韵, 刘庆昌, 李惟基. 甘薯育种学[M]. 北京：中国农业大学出版社,1998,,19-38
    [25] 闫华超, 高 岚, 李桂兰. 分子标记技术的发展及应用[J]. 生物学通报, 2006, 41(2) : 17-19.
    [26]Russell J R, Fuller J D, Macaulay M, Hatz B G, Jahoor A, Powell W,Waugh R. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theoretical and Applied Genetics, 1997, 95: 714-722.
    [27] Pejic I, Ajmone-Marsan P, Morgante M, Kozumplick V, Castiglioni P,Taramino G, Motto M. Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs and AFLPs. Theoretical and Applied Genetics, 1998, 97: 1248-1255.
    [28] 郭起荣,任立宁,牟少华, 等. 毛竹种质分子鉴别SRAP、AFLP、ISSR联合分析[J]．江西农业大学学报,2010,32(5):982-986
    [29] Fu X P, Ning G G, Gao L P, Bao M Z．Genetic diversity of Dianthus accessions as assessed using two molecular marker systems (SRAPs and ISSRs)and morphological traits[J]. Sci Hort, 2008, 117:263-270
    [30] 陶爱芬, 祁建民, 粟建光,等. SRAP和ISSR及两种方法结合在分析黄麻属起源与演化上的比较[J]. 作物学报, 2011,37(12):2277-2284
    [31] 陶爱芬, 祁建民, 粟建光,等. 黄麻种质资源遗传多样性的SRAP分析[J]. 植物科学学报, 2012, 30( 2) :178 -187
    [32] 刘建丰,王志德,刘艳华,牟建民. 应用SRAP标记研究烟草种质资源的遗传多样性[J].中国烟草科学,2007,28(5):49-53
    [33] 许先松,刘志钦,林晓丹, 等. 基于形态及SRAP标记的辣椒资源遗传多样性及亲缘关系比较[J].福建农林大学学报, 2011,40(1):48-53
    [34] 卢启泉. 四类平菇种质资源的分子标记分析[D]. 福州: 福建农林大学,2004
    [35] 应正河. RAPD、SRAP和ISSR标记在香菇种植资源的应用及其SCAR标记的建立[D]. 福州: 福建农林大学, 2006
    [36] 罗凯, 卢会翔, 吴正丹, 等. 中国西南地区甘薯主要育种亲本的遗传多样性及群体结构分析[J]. 中国农业科学, 2016, 49(3):593-60
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History
  • Received:October 12,2017
  • Revised:March 25,2018
  • Adopted:November 15,2017
  • Online: May 08,2018
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