Peanut pod rot is a fungal disease worldwide that directly affects the production and quality of peanut. In order to identify resistant germplasm against pod rot for peanut breeding, 116 accessions of peanut, including 77 American and 39 domestic germplasm, were evaluated in a two-year field experiment in this study. The results indicated that an extremely significant difference for peanut injury index was observed among the tested peanut accessions (P<0.01) and a significant difference was also found for injury index between 2016 and 2017 years (P<0.05) via variance analysis. Pod rot resistance of tested peanut accessions could be divided into 5 groups according to the cluster analysis of injury index. There were high resistance (HR), resistance (R), medium resistance (MR), susceptible (S) and high susceptible (HS). The result could be used as a resistance criterion to clearly evaluate the pod rot resistance of peanut. A total of 72 accessions were consistent in pod rot resistance in 2 years evaluation, including 2 highly resistant materials (HR) with average injury indexes of 16.67 and 21.91, and 4 resistant materials (R) with average injury indexes of 34.79, 26.33, 39.98 and 42.37, and 19 medium resistant materials (MR) with injury indexes from 45.19 to 54.67, and 42 susceptibility materials (S) with injury indexes from 60.18 to 74.60, and 5 high susceptibility materials (HS) with average injury indexes of 75.87, 77.83, 78.57, 80.74 and 81.11, respectively. The results could provide reference for evaluation of pod rot resistance and favorable materials for genetic improvement in peanut resistant breeding program.