Cloning and Expression analysis of F-box gene SLY1 in Vitis vinifera L.

doi:10.13430/j.cnki.jpgr.20180111001

Home > Archive>Volume 19, Issue 5, 2018 >959-966. DOI:10.13430/j.cnki.jpgr.20180111001

Cloning and Expression analysis of F-box gene SLY1 in Vitis vinifera L.
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                        10.13430/j.cnki.jpgr.20180111001
                    
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Affiliation:Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,Institute of Pomology, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014
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Abstract:

SLEEPY1(SLY1), one of the main elements in SCF complex, belongs to F-box protein. It plays an important regulatory role in the signal transduction process of gibberellin. A full length of F-box gene, designated VvSLY1, was isolated from grapevine ‘Summer Black’ (Vitis vinifera L.) by electronic cloning and RT-PCR method. The gene was 1096 bp and contained a complete open reading frame of 555 bp that encoded 184 amino acids. Sequence alignment and motif analysis suggested that the VvSLY1 gene contained LGG and LSL, which were conserved domains of F-box protein. Phylogenetic tree analysis showed that the VvSLY1 protein had the closest genetic relationship with the Populus euphratica protein. Results of qRT-PCR revealed that the expression of VvSLY1 was up-regulated by gibberellin treatment in the fast expansion development of fruit.

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Received:January 11,2018
Revised:April 14,2018
Adopted:May 14,2018
Online: September 14,2018
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