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Home > Archive>Volume 19, Issue 4, 2018 >731-739. DOI:10.13430/j.cnki.jpgr.20180124001
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Molecular Isolation and Expression Analysis of ScHAK9 Gene from Sugarcane
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1Guangxi Crop Genetic Improvement and Biotechnology Laboratory Nanning 530007 ;,1Guangxi Crop Genetic Improvement and Biotechnology Laboratory Nanning 530007 ;,1Guangxi Crop Genetic Improvement and Biotechnology Laboratory Nanning 530007 ;,2Sugarcane Research Institute Guangxi Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi)，Ministry of Agriculture, Nanning 530007,1Guangxi Crop Genetic Improvement and Biotechnology Laboratory Nanning 530007 ;,2Sugarcane Research Institute Guangxi Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi)，Ministry of Agriculture, Nanning 530007,2Sugarcane Research Institute Guangxi Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi)，Ministry of Agriculture, Nanning 530007,1Guangxi Crop Genetic Improvement and Biotechnology Laboratory Nanning 530007 ;,1Guangxi Crop Genetic Improvement and Biotechnology Laboratory Nanning 530007 ;

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    Abstract:

    The KUP/HAK/KT family gene plays an important role in regulation of potassium accumulation. In this research, we isolated the complete coding sequence of ScHAK9 and investigated the transcriptional levels in ROC22. ScHAK9 gene was composed of 2352 nucleotides that encodes 783 amino acids with 12 predictable transmembrane domains. The deduced protein was predicted to be a basic dewatering protein and localized to the cytoplasmic membrane. The secondary structure consisted of alpha spiral structure, irregular curling structure and extended long chain. ScHAK9 gene showed highly sequence identity with the homologous genes as compared with ZmHAK9 gene(Zea mays L). Real-Time PCR showed that the ScHAK9 gene highly expressed in the leaves, followed by the stem and the root at last. ScHAK9 gene expression was induced under drought stress, and the higher expression decreased after re-watering. Thus, our work provided first insight about ScHAK9, which might play a role in drought stress resistance.

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History
  • Received:January 24,2018
  • Revised:May 22,2018
  • Adopted:May 14,2018
  • Online: July 16,2018
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