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Home > Archive>Volume 19, Issue 5, 2018 >942-950. DOI:10.13430/j.cnki.jpgr.20180131002
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Genetic Analysis and Mapping of a Yellow-green Leaf Mutant of Tobacco (Nicotiana tabacum L.)
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1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,3China Tobacco Hunan Industrial Co., Ltd., Changsha 410007,3China Tobacco Hunan Industrial Co., Ltd., Changsha 410007,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;

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    Abstract:

    In this study, F<sub>1</sub> population was constructed by a cross derived from tobacco cultivars G117 and RG13. A natural mutant individual was found in F<sub>3</sub> lines during breeding selection. The mutant exhibits a yellow-green leaf phenotype, which is controlled by one recessive gene by classical genetic analysis. Significant markers related with aimed trait were detected by jointly 430 K SNP chip with two extreme selective populations. Subsequently, the entire segregation population derived from one F<sub>7</sub> plant was genotyped using PCR method at these significant markers loci. Finally, the recessive gene controlled a yellow-green leaf phenotype in tobacco was mapped to chromosome 5 and co-segregated with the SNP marker M7. These results could enhance our understanding of the inheritance of yellow-green leaf mutant, and provide a new avenue for mapping the mutant loci or the QTL in tobacco by combining SNP chip and extreme selection populations.

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History
  • Received:January 31,2018
  • Revised:June 23,2018
  • Adopted:May 21,2018
  • Online: July 05,2018
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