2025-4-6- 16
Home > Archive>Volume 19, Issue 5, 2018 >942-950. DOI:10.13430/j.cnki.jpgr.20180131002
PDF HTML XML Export Cite reminder
Genetic Analysis and Mapping of a Yellow-green Leaf Mutant of Tobacco (Nicotiana tabacum L.)
Author:
Affiliation:

1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,2Zhenzhou Tobacco Research Institute of CNTC, Zhengzhou 563006;,3China Tobacco Hunan Industrial Co., Ltd., Changsha 410007,3China Tobacco Hunan Industrial Co., Ltd., Changsha 410007,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;,1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao Shandong 266100;

  • Article
    • | |
  • Metrics
    • |
  • Reference [24]
    • | | | |
  • Comments
    Abstract:

    In this study, F<sub>1</sub> population was constructed by a cross derived from tobacco cultivars G117 and RG13. A natural mutant individual was found in F<sub>3</sub> lines during breeding selection. The mutant exhibits a yellow-green leaf phenotype, which is controlled by one recessive gene by classical genetic analysis. Significant markers related with aimed trait were detected by jointly 430 K SNP chip with two extreme selective populations. Subsequently, the entire segregation population derived from one F<sub>7</sub> plant was genotyped using PCR method at these significant markers loci. Finally, the recessive gene controlled a yellow-green leaf phenotype in tobacco was mapped to chromosome 5 and co-segregated with the SNP marker M7. These results could enhance our understanding of the inheritance of yellow-green leaf mutant, and provide a new avenue for mapping the mutant loci or the QTL in tobacco by combining SNP chip and extreme selection populations.

    Reference
    [1]Wu H, Zhang L X. The PPR protein PDM1 is involved in the processing of rpoA pre-mRNA in Arabidopsis thaliana[J]. China Sci Bulletin, 2010, 55(30): 3485-3489
    [2]Gong X D, Jiang Q, Xu J L, et al. Disruption of the rice plastid ribosomal protein S20 leads to chloroplast developmental defects and seedling lethality[J]. G3-Genes Genom Genet, 2013, 3(10): 1769-1777
    [3]韩帅,王立静,钟世宜,等.一个新的玉米叶色突变体的遗传分析及基因定位[J].玉米科学, 2012,20(03):26-28
    [4]金婷.大麦白化颖壳突变体的遗传分析和基因定位[D].杭州:浙江师范大学化学与生命科学学院, 2013
    [5]程旭东.番茄的硫胺素合成酶基因LeTHIC的克隆及功能分析[D].北京:中国科学院遗传与发育研究所, 2008
    [6]李娜娜.新梢白化茶树生理生化特征及白化分子机理研究[D].杭州:浙江大学, 2015
    [7]Wu Q Z, Wu X R, Zhang X F, et al. Mapping of two white stem genes in tetraploid common tobacco[J]. Mol Breeding, 2014, 34(3): 1065-1074
    [8]史琳,陈彦.植物白化基因的作用机制与研究进展[J].安徽农业科学, 2017,45(12):132-135
    [9]Lander E S. The new genomics: global views of biology[J]. Science, 1996, 274: 536
    [10]吴秋红,陈永兴,李丹,等.利用SNP芯片和BSA分析规模化定位小麦抗白粉病基因[J/OL].作物学报, 2017,1-15[2017-12-24].http://kns.cnki.net/kcms/detail/11.1809.S.20171027.1736.008.html
    [11]Julio E, Denoyes-Rothan B, Verrier J L, et al. Detection of QTLs linked to leaf and smoke properties in Nicotiana tabacum based on a study of 114 recombinant inbred lines[J]. Mol Breeding, 2006, 18: 69-91
    [12]柴利广.白肋烟遗传连锁图谱的构建及烟碱含量QTL的定位[D].武汉:华中农业大学, 2008
    [13]李华丽,陈美霞,周东新,等.烟草六个重要性状的QTL定位[J].作物学报, 2011,37(9):1577-1584
    [14]童治军,焦芳婵,吴兴富,等.烤烟6个农艺性状的QTL定位[J].作物学报, 2012,38(8):1407-1415
    [15]张吉顺,王仁刚,杨春元,等.国内外烤烟品种农艺性状的遗传多样性及与SRAP标记的关联分析[J].作物学报, 2012,38(6):1029-1041
    [16]Bai D, Reeleder R, Brandle J E. Identification of two RAPD markers tightly linked with the Nicotiana debneyi for resistance to black root rot of tobacco[J]. Theor Appl Genet, 1995, 91(8): 1184-1189
    [17]焦天雷.烟草(Nicotiana tabacum L.)赤星病抗性QTL的定位分析[D].杭州:浙江大学, 2011
    [18]朱承广,任民,蒋彩虹,等.以关联分析发掘烟草抗赤星病基因分子标记[J].中国烟草科学, 2017,38(1):68-72
    [19]张剑锋,罗朝鹏,何声宝,等.应用SNP标记分析24份烟草品种的遗传多样性[J].烟草科技, 2017,50(11):1-8
    [20]Bindler G, Plieske J, Bakaher N, et al. A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development[J].Theor Appl Genet, 2011, 123: 219-230
    [21]VanOoijen J W. JoinMap 4, Software for the calculation of genetic linkage maps in experimental populations[M]. Netherlands: Wageningen, 2006
    [22]Voorrips R E. MapChart: software for the graphical presentation of linkage maps and QTLs[J]. J Hered, 2002, 93: 77-78
    [23]唐立群,肖层林,王伟平.SNP分子标记的研究及其应用进展[J].中国农学通报, 2012,28(12):154-158
    [24]柴利广,张俊杰,林国平,等.白肋烟两个DH群体的构建与染色体倍性的鉴定[J].中国烟草学报, 2007(02):33-37
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

Copy
Share
Article Metrics
  • Abstract:1459
  • PDF: 2432
  • HTML: 0
  • Cited by: 0
History
  • Received:January 31,2018
  • Revised:June 23,2018
  • Adopted:May 21,2018
  • Online: July 05,2018
Article QR Code
You are the 606148th visitor 京ICP备09069690号-23
® 2025 All Rights Reserved
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
Firefox, Chrome, IE10, IE11 are recommended. Other browsers are not recommended.