Enhancing Rice Resistance to Bacterial Blight by Genome Editing

doi:10.13430/j.cnki.jpgr.2018.03.018

Home > Archive>Volume 19, Issue 3, 2018 >523-530. DOI:10.13430/j.cnki.jpgr.2018.03.018

Enhancing Rice Resistance to Bacterial Blight by Genome Editing
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                        10.13430/j.cnki.jpgr.2018.03.018
                    
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Affiliation:National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS,National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS,National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS,National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS,National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS,National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS,National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS,National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS,National Key Facility for Crop Gene Resources and Genetic Improvement NFCRI,Institute of Crop Sciences,Chinese Academy of Agriculture Sciences CAAS
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Abstract:

Bacterial blight of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most destructive diseases of rice, and seriously affects the rice production in Asian countries. Utilizing resistance genes to breed resistant varieties is the most effective, economic and environment-friendly strategy for disease control. W6023 is a resistant introgression line which was generated by a cross between a wild rice accession and IR24. In our previous work, Pong2-1(Os02g20780)and Pong11-1 (Os11g14160)display transcriptional activation in IR24, but not in resistant line W6023. And Pong2-1 and Pong11-1 are highly similar in DNA sequences, but show different forms of transcripts. To investigate whether these two genes are susceptibility factors to Xoo, we knocked out Pong2-1 and Pong11-1 in IR24 using CRISPR/Cas9 methods and obtained a series of mutant plants. Inoculation assays showed the double mutant lines show enhanced resistance to PXO99_A comparing with the wild type.

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Received:February 04,2018
Revised:March 15,2018
Adopted:March 10,2018
Online: May 08,2018
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